Research 2 | Beckstein Lab

. . . . .
Molecular mechanism of ligand recognition by the Mhp1 transporter

Molecular mechanism of ligand recognition by the Mhp1 transporter

The hydantoin transporter Mhp1 is a sodium?coupled secondary active transport protein of the nucleobase?cation?symport family and shares the widespread 5?helix inverted repeat transporter architecture. Our previous work showed Mhp1 functions according to the alternating access mechanism. In our new paper in EMBO J , we elucidate detailed events of substrate binding, through a combination of crystallography, molecular dynamics, site?directed mutagenesis, biochemical/biophysical assays, and the design and synthesis of novel ligands.

Comment Continue Reading →
List of Publications

List of Publications

List of publications of of the lab (since 2012) and Oliver Beckstein (2001–2011).

Comment Continue Reading →
AdK apo PMF

AdK apo PMF

The enzyme adenylate kinase (AdK) undergoes a large hinge-like motion. In 2009, we studied the conformational transition between open and closed E. coli AdK without substrate, i.e. “apo AdK”, with a variety of computational methods. As part of the study we also produced a free energy landscape (a potential of mean force or PMF) as a function of the two domain angles. Here we make the data of the underlying free energy landscape available to other researchers so that they can use them in their own research.

Comment Continue Reading →